Nucleus Rim Measurement
Measurement of intensity changes at the periphery of nucleus over time
Input: 2-channel image sequence, a channel with DAPI and a channel with NPC (Nuclear Pore Complex) protein signal.
Output: Intensity vs time plot.
Measures changes in NPC intensity at nuclear periphery over time. DAPI channel is used for the segmentation of “nuclear periphery” using binarization and subtraction between dilated and eroded binary image.
Fiji (Jython). If ImageJ is added with Jython library, it works also with legacy ImageJ.
Download the script nucRim2 and execute the jython script.
Input file should be in hyperstack format and opened as an active image stack.